Department of Microbiology, Istanbul University, Turkey.
Title: Prevelance of occult HBV in chronic hepatitis C and cryptogenic hepatitis patients
Bulent Cakal completed his PhD from istanbul University, Turkey. He worked Post-doctoral Research Fellow about molecular virology of Hepatitis B Virus (HBV) at Department of Microbiology and Immunology College of Medicine University of Illinois at Chicago / U.S.A. He has three publication over 20 times, and his publication H-index is 6.98 and he has worked department of medical microbiology and he has two research project about HBV.
Occult Hepatitis B Virus (HBV) infection (OBI) is considered as the possible a phase of the HBV natural history but it remains unclear the molecular mechanisms and clinical impact and epidemiology aspect of OBÄ° (1,2,3). We investigated the prevalence of OBI and its clinical impact among patients with Hepatitis C virus (HCV) infection and with cryptogenic hepatitis. This study protocol was approved by the ethics committee of Ä°stanbul University Ä°stanbul School of Medicine (No: 2015/1519). This prospective cohort study included a total of 60 HBsAg-negative patients (27 patients with chronic HCV and 33 patients with cryptogenic hepatitis) were enrolled in department of gastroenterology, Istanbul Faculty of Medicine. Liver tissue samples had been obtained by percutaneous needle liver biopsy and immediately frozen and stored at -80°C. Total nucleic acids were extracted from frozen liver biopsies using QIAamp DNA Mini Kit (Qiagen) according to the manufacturer’s instructions. OBI was defined as HBV DNA positivity in 2 or more different viral genomic regions by nested polymerase chain reaction PCR using 4 sets of primers in preS-S (S), precore-core (C), Pol, and X viral regions of the HBV genome. Plasmid HBV DNA 4.1 kb and liver biopsy samples obtained from patients with chronic HBV infection (positive control) were used. Statistical analyses were evaluated using Mann–Whitney, Chi-square test and Kruskal Wallis tests. The baseline characteristics of patients are presented in Table 1. The prevalence of OBI was %25.9 (7/26) with %27.3 (9/33), %26.7 (16/60) in patients anti-HCV (+),cryptogenic hepatitis, and totaly respectively (Table 2). There wasn’t significant differences for prevelance of OBÄ° between patients with Chronic HCV infection and cryptogenic hepatitis (P=0.907). Patients with anti-HCV (+), OBÄ° (+) were older compared with patients anti-HCV (+), OBÄ° (-), (P: 0.033). As it is expected that cryptogenic hepatitis patients had higher serum alkaline phosphatase and gamma-glutamyltransferase level (P<0.05). Clinical signifance and role of OBI in patients with chronic HCV infection is controversial (4,5,6). According first results of the study to prevelance of OBÄ° is correlated with endemicity of Hepatitis B infection moreover OBÄ° can be associated with liver injury rather than chronic HCV infection. Therefore, it appears that host factors rather than viral factors are more responsible for OBI.